Evaluation of dog semen quality after slow (biological freezer) or rapid (nitrogen vapours) freezing
نویسندگان
چکیده
منابع مشابه
Evaluation of dog semen quality after slow (biological freezer) or rapid (nitrogen vapours) freezing.
Three ejaculates were collected from each of five dogs. After initial evaluation, the sperm-rich fractions were diluted to 100 x 10(6) spermatozoa x mL(-1) in two steps with an egg yolk-TRIS extender containing a final concentration of 5% glycerol and 0.5% Equex STM paste. Half of the 0.5 mL straws obtained from each ejaculate were frozen on nitrogen vapours (4 cm above the liquid surface) ("ra...
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The purpose of this study was to determine the negative effects (cryodamage) on human spermatozoa after freeze-thawing and to determine whether freeze-thawing of spermatozoa with a programmed slow freezer is better than freezing with liquid nitrogen vapour (rapid freezing) with regard to alterations in sperm chromatin and morphology in semen from fertile (donor) and subfertile, IVF/ICSI, patien...
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ژورنال
عنوان ژورنال: Reproduction Nutrition Development
سال: 2005
ISSN: 0926-5287,1297-9708
DOI: 10.1051/rnd:2005002